Table 1 PCR conditions used for each target group of soil biota
From: Effect of fungal, oomycete and nematode interactions on apple root development in replant soil
| PCR step | Target group (marker region) | ||||
|---|---|---|---|---|---|
| Bacteria (16S, V4 region) | Fungi (18S, ITS1 and 2 regions) | Oomycetes (18S, ITS1 region) | Nematode (18S small subunit) | ||
| Initial denaturation | 95 °C, 5 min | 95 °C, 5 min | 95 °C, 5 min | 95 °C, 5 min | |
| No. PCR cycles | 25 | 30 | 15 | 30 | |
| Denaturation | 94 °C, 30 s | 94 °C, 30 s | 94 °C, 30 s | 94 °C, 45 s | |
| Annealing | 52 °C, 60 s | 55 °C, 60 s | 52 °C, 60 s | 57 °C, 45 s | |
| Elongation | 72 °C, 60 s | 72 °C, 60 s | 72 °C, 60 s | 72 °C, 90 s | |
| Final elongation | 72 °C, 7 min | 72 °C, 7 min | 72 °C, 7 min | 72 °C, 7 min | |